Pictures related to liposomes and drug delivery technology will be posted to this board. NK and macrophages were assessed using flow cytometry. NK cell and macrophage depletion were conducted using anti‐asialo GM1 and clodrosome, respectively. Each Clodrosome Macrophage Depletion Kit contains Clodrosome and an equal volume of control liposomes. Injection, Publications Not Specifying Route of Administration, Contain unmodified multilamellar liposomes similar to those used in most published depletion studies, Liposome size range (0.3-3 µm) is optimal for immediate phagocyte recognition and uptake, Kits contain equal volumes of each reagent packaged in stoppered glass vials with multidose vial adapters, Select the Fluorescent Kit to visualize control liposomes, Clodronate encapsulated inside multilamellar liposome aqueous spaces, Extruded through 2 µm membranes to ensure that liposomes easily pass through lung capillaries, Prepared and packaged under sterile conditions, Prepared identically to Clodrosome except that clodronate is not added, Required control since phagocytes can respond to liposome uptake, Prepared identically to Clodrosome (without clodronate; with fluorophore), May be tracked by fluorescence methods (confocal microscopy, FACS, etc. Jul 20, 2013 - Liposomal clodronate therapy is a very useful drug formulation for treating autoimmune hemolytic anemia. 74, no. The product is suitable for dosing with various routes including intravenous, intraperitoneal, subcutaneous, intranasal, intratracheal and etc. Injection, Publications Reporting Spleen Macrophage Depletion by I.V. Macrophage depletion also reduces hyperplasia of vein grafts. The kits come in 2 different varieties: standard kits and mannosylated kits (mainly to target central … macrophage depletion kit Clodrosome a nd Encapsome (Encapsula NanoSciences). Clodronate molecules are encapsulated in small MLV liposomes for superior activity, physical and chemical stability, and convenience of use and handling. Several strategies were taken to verify the contribution of M2 macrophage and CHI3L1: macrophage depletion by clodrosome, local CHI3L1 inhibition using intravitreally injection neutralize antibody (mAY), and depletion of CHI3L1 receptor (IL13-Ra2) by small-interfering RNA (siRNA). Depletion of macrophages by clodrosome resulted in reduction of LPS-induced cytokine generation and alleviated LPS-induced thrombocytopenia. Thus, macrophage depletion has potential as a novel cancer therapy and positive responses have been reported in rodent tumor models. The GS system is discussed in whole or part in connection with EP 0 216 846, EP 0 256 055, EP 0 323 997 and EP 0 338 841 and U.S.P.N.s 5,591 ,639 and 5,879,936. Mannosylated fluorescent liposomes w/ DiI. Hi, did anyone use the Clodrosome® standard macrophage depletion kit (containing Clodronate liposome and control liposomes), and know how to prepare it … To deplete macrophages, 2.5 ml clodrosome (Liposomal Clodronate, 5 mg/ml, Clodrosome Macrophage Depletion kit, Encapsula Nano Science) were injected into a rat through the tail vein. The homing of MM PC to specific niches within the BM that support their colonization and growth is a critical event in the establishment of MM tumors , .As there was no effect on MM PC proliferation or survival following clo-lip treatment, we next investigated the effect of clo-lip–mediated macrophage depletion on 5TGM1 MM PC homing and retention. Clodrosome® is a multilamellar liposome suspension in which clodronate is encapsulated in the aqueous compartments of the liposomes. The code will save the url of this webpage to the device for mobile sharing and storage. Radiation-induced lung fibrosis (RIF) is a delayed side-effect of chest radiotherapy, frequently associated with macrophage infiltration.We aimed to characterise the role of pulmonary macrophages in RIF using human lung biopsies from patients receiving radiotherapy for thorax malignancies and a RIF model developed in C57BL/6 mice after 16-Gy thorax irradiation.High numbers of macrophages … Mannosylated macrophage depletion kit is composed of one vial of m-Clodrosome® … Micron sized liposomes engineered specifically to target macrophages in different tissues and organs. m-Fluoroliposome fluorescent control liposomes (Mannosylated Fluorescent Clodrosome Kit) described below. The clodronate-containing liposomes … To determine whether depletion of alveolar macrophages was depend on encapsulation of clodronate in liposomes, the effects of plain PBS liposomes and free and non-encapsulated clodronate on tracheal insufflation was studied by Breg et al (Journal of Applied Physiology, June 1993, vol. Select either m-Encapsome control liposomes (Mannosylated Clodrosome Kit) or. These kits are also available with various fluorescent control reagents such as DiI (ex/em: 549/565 nm), DiO (ex/em:484/501 nm), DiD (ex/em: 644/665 nm), … Control rats were injected with the same amount of encapsome (control liposomes). Manufacturer: ENCAPSULA NANAOSCIENCE LLC CLD89012ML Standard macrophage depletion kit- The kit is composed of one 2 ml vial of Clodrosome (Clodronate liposomes) and one 2 ml vial of Encapsome (plain liposomes for control). The liposomes are filtered through 2 μm polycarbonate membranes to ensure that the larger particles, which may be toxic … NK cell and macrophage depletion were conducted using anti‐asialo GM1 and clodrosome, respectively. Clodrosome; Encapsome; Fluoroliposome-DiI; Fluoroliposome-DiO; Fluoroliposome-DiD; ... Full macrophage depletion kit (w/Fluoroliposome®-DiO) $ 280.00 – $ 2,275.00 Select options $ 280.00 – $ … For … Standard Macrophage Depletion Kits. The kits come in 2 different varieties: standard kits and mannosylated kits (mainly to target central nervous system). Clodrosome Depletion of Macrophages Enhances OV Efficacy (A) Treatment regimen of A673 tumor xenograft-bearing mice given 400 μg of liposome or Clodrosome intravenously (i.v.) Required fields are marked *, Select quantity or request quotation for larger quantities, (composed of one vial of m-Clodrosome® and one vial of m-Encapsome®), Copyright © 2018 Encapsula NanoSciences LLC, Intracranial (intracisternal, Intracerebroventricular), Publications Citing Intracerbroventrivular (ICV) Administration into the CSF, Publications Citing Intraventricular (ICV) Administration into the CSF, Publications Citing Intracisternal Administration into the CSF, Publications Reporting Intrahippocampal Administration, Publications Reporting Intradermal Injection, Publications Reporting Aerosol Administration, Publications Reporting Intranasal Administration (Intrapulmonary), Publications Reporting Intratracheal Administration, Publications Reporting Pharyngeal Administration, Be the first to review “Standard macrophage depletion kit”. Standard macrophage depletion kit $ 200.00 – $ 1,650.00 (composed of one vial of Clodrosome® and one vial of Encapsome®) Copyright © 2018 Encapsula NanoSciences LLC, Intracranial (intracisternal, Intracerebroventricular), Publications Citing Intracerbroventrivular (ICV) Administration into the CSF, Publications Citing Intraventricular (ICV) Administration into the CSF, Publications Citing Intracisternal Administration into the CSF, Publications Reporting Intrahippocampal Administration, Publications Reporting Intradermal Injection, Publications Reporting Aerosol Administration, Publications Reporting Intranasal Administration (Intrapulmonary), Publications Reporting Intratracheal Administration, Publications Reporting Pharyngeal Administration, DiI (lipophilic fluorescent dye: ex/em~550/580 nm), Suspended in Phosphate Buffered Saline at pH 7.4, DiO (lipophilic fluorescent dye: ex/em~484/501 nm), m-Fluoroliposome®-DiD (Mannosylated Fluorescent Control Liposomes), DiD (lipophilic fluorescent dye: ex/em~648/680 nm), Mannosylated Fluorescent Control Liposomes, Publications Reporting Intra-articular Administration, Publications Reporting Intracerbroventricular Administration, Publications Reporting I.V. Your email address will not be published. ). Adminstration by Retro-orbital Injection, Publications Reporting Monocyte Depletion by I.V. To investigate the effectiveness of this approach in dogs with cancer, we evaluated the effects of the macrophage depleting agent liposomal clodronate (LC) in dogs with soft tissue sarcoma (STS). NC0302518 $248.00 / Each Injection, Publications Reporting Systemic Macrophage Depletion by I.V. Macrophage depletion has been used extensively to study autoimmune disease and more recently in tumor models. Immunohistochemistry for tissues (PD-L1, caspase-3, Ki-67, NK cells, macrophages), and Western blotting for OS lung tumors (p-Stat3, p-Erk1/2) was performed. Clodronate, and Encapsom e is the Control Liposomes. PD‐L1 expression was observed in human OS cells and OS patient lung metastases. Catalog No. Macrophage Depletion Kit (Standard) Each standard macrophage depletion kit contains one vial of Clodronate encapsulated liposomes (Clodrosome) and one vial of plain liposome for control (Encapsome) with the same lipid composition as Clodrosome. Apply slight downward pressure until the syringe ring "snaps" into place. PD‐L1 expression was observed in human OS cells and OS patient lung metastases. Use this unique QR (Quick Response) code with your smart device. Another preferred expression system for the development of stable cell lines is the Freedom ™ CHO-S Kit (Life Technologies). In summary, we revealed that M1/M2 imbalance contributes to the aggravated cardiomyopathy in ovariectomized diabetic mice, and therapeutically reducing miR155 in macrophages by AuNP serves as a promising strategy in improving cardiac function. To edit this sidebar, go to admin backend's. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics Read More See FAQ (Question 1) for more information on the use of fluorescent liposomes. The restoration efficacy of miR155-AuNP was much better than general macrophage depletion by clodrosome. Injection, Publications Reporting Spleen/Liver Macrophage Depletion by I.V. Clophosome®-A composed of anionic lipids further improve the efficacy in macrophage depletion to above 90% in spleen after a single intravenous injection. Macrophage depletion also remarkedly diminished large platelet aggregate formation in the splenic marginal zone but had less effect on those in red pulp. on … Macrophage depletion. on day −3 and intraperitoneally (i.p.) 62812-2819). Mannosylayed control liposomes complete the mannosylated Clodrosome Macrophage Depletion Kit. Standard Clodrosome Reagents. This particular autoimmune disease destroys red blood cells. NK cell and macrophage depletion were conducted using anti-asialo GM1 and clodrosome, respectively. Macrophage depletion kits are composed of two vials; one vial of Clodrosome® … In Vivo M2 Macrophage Depletion Analysis M2 macrophages were depleted from FNI rats by using the Mannocylated‐Clodrosome Macrophage Depletion Kit (SKU # 8901, Encapsula NanoSciences LLC, Nashville, TN, http://www.encapsula.com). Home → Clodrosome → Standard macrophage depletion kit, (composed of one vial of Clodrosome® and one vial of Encapsome®), 2 ml – $200.00, 5 ml – $400.00, 10 ml – $700.00, 15 ml – $1000.00, 20 ml – $1350.00, 25 ml – $1650.00, Your email address will not be published. Encapsome® is formulated and prepared identically to Clodrosome® except that clodronate is not added to the liposomes. … Place the syringe ring on the plunger (slit facing the plunger). Contact Encapsula NanoSciences LLC 5409 Maryland Way Suite 360 Brentwood, TN 37027 Phone: 615-884-4442 Fax: 615-250-8747 URL: www.encapsula.com Mannosylated Clodrosome Macrophage Depletion Kits Contain unmodified multilamellar liposomes similar to those used in most published depletion studies 4-Aminophenyl-alpha-D- Mannopyranoside incorporated into the liposomes Liposome size range (0.3-3 µm) is optimal for immediate phagocyte recognition and uptake Clodrosome is a Liposomal . Encapsula Nano S ciences Clodrosome® Macrophage Depletion Kit Product Information Animals systemically dosed with Clodrosome will become immune-compromised immune-compromised. A human LM7 OS mouse model was used. Home → Shop → Mannosylated Macrophage Depletion Kits. Remove the components of the administration kit from their respective pouches and place onto the sterile field.-Withdraw the syringe plunger approximately 1 inch. Encapsula NanoSciences | This is the pinterest site for Encapsula NanoSciences. Title: Macrophage Depletion Reagents (Clodrosome®) - Encapsula NanoSciences- Manufacturer of liposome based kits and formulations for research laboratories Description: Micron sized liposomes engineered specifically to target macrophages in different tissues and organs. -Prepare a sterile field. Further details on each reagent is available by clicking on “More Info” below its image. Usual therapies include the use of corticosteroids and splenectomy, but this one gives very fast and promising results by destroying macrophages. We have achieved 80-90% macrophage depletion in spleen (red pulp macrophages) 24 hours after a single 0.2 mL intravenous injection.
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